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BCOP Applications


BCOP is primarily used to evaluate test materials in the moderate to extreme irritancy/corrosive range. However, exposure protocol modifications can be made to increase the sensitivity of the assay system to resolve among milder ingredients and formulations.

Test Material Compatibility

The assay is compatible with both water soluble and insoluble formulations. Both solid and liquid test materials can be tested. Since test materials are topically applied, the test system is suitable for creams, pastes, highly viscous materials, and powders.

Alternate Endpoints

BCOP’s multiple endpoints allow the investigations of mechanism of action. An additional histological endpoint can be added to assess the types of observed lesions and the depth and degree of injury. Based upon the work of Maurer and Jester (2002), the depth and degree of injury may be used to predict the severity and outcome of the corneal injury.

Test article concentrations and exposure times may be adjusted as necessary to accommodate specific physical characteristics or client needs. Specialized protocols may be prepared as requested through consultation with the Study Director.


• In the US and in Europe, the BCOP assay has been approved for identification of corrosive substances and those chemicals not requiring classification according to UN GHS.

• OECD Test Guideline was adopted (TG437) in 2009 and revised in 2013.

• The US EPA accepts the BCOP assay as part of a tiered strategy for the labeling of cleaning products with anti-microbial claims.

Ocular Irritation Assays - Continuum of Sensitivity